Human MesP1
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| Cat-Nr. | 300-060 |
| Size | 10 µg |
| Price | 210 € |
| Source | Insect cells |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 268 |
| Molecular Weight | 34 kDa |
| Biological Activity | Testing in progress. |
| Species Reactivity | Human |
| Buffer | 0.5X PBS |
| Reconstitution | Human MesP1 should be reconstituted in sterile water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C. |
| Stability and Storage | The lyophilized human MesP1, though stable at room temperature, is best stored desiccated below 0°C. Freeze/thaw cycles will result in significant loss of activity. |
| Synonyms | MESP1; bHLHc5 |
| Description | ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis. It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation. MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1. |
| Protein Sequence | MAQPLCPPLSESWMLSAAWGPTRRPPPSDKDCGRSLVSSPDSWGSTPADSPVASPARPGTLRDPRAPSVGRRGARSSRLGSGQRQSASEREKLRMRTLARALHELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEESLQRRCRQRGDAGSPRGCPLCPDDCPAQMQTRTQAEGQGQGRGLGLVSAVRAGASWGSPPACPGARAAPEPRDPPALFAEAACPEGQAMEPSPPSPLLPGDVLALLETWMPLSPLEWLPEEPK |
| Uniprot ID | Q9BRJ9 |
| Protein RefSeq | NP_061140.1 |
| mRNA RefSeq | NM_018670.3 |
Figures
SDS-PAGE analysis of recombinant human MesP1. Sample was loaded in 15% SDS-polyacrylamide gel and stained with Silver staining.
Lane 1: Molecular weight marker (kDa); Lane 2: reduced MesP1.
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