Human PDGF-AA
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| Cat-Nr. | 200-051S |
| Size | 2 µg |
| Price | 65 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 125 |
| Molecular Weight | 28.5 kDa |
| N Terminal Sequence | SIEEAVPA |
| Endotoxin Levels | < 0.1 ng per ug of PDGF-AA |
| Biological Activity | The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts). |
| Species Reactivity | Human |
| Buffer | 50mM acetic acid |
| Reconstitution | Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we recommend to add at least 0.1% human or bovine serum albumin. |
| Stability and Storage | The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles. |
| Synonyms | PDGFA; PDGF1; PDGF-A |
| Description | PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids). |
| Protein Sequence | SIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT |
| Uniprot ID | P04085 |
| Protein RefSeq | NP_002598.5 |
| mRNA RefSeq | NM_002607.5 |
Figures
SDS-PAGE analysis of recombinant human PDGF-AA. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and visualized with Silver stain.
Dose-dependent stimulation of cell proliferation in NHDF cells by recombinant human PDGF-AA. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
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