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Human IL-6

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Cat-Nr.200-030
Size5 µg
Price65 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE
Length [aa]186
Molecular Weight21.1 kDa
N Terminal SequenceMAPVPPGE
Endotoxin Levels< 0.1 ng per µg (IEU/µg) of rh IL-6
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of murine hybridoma B9 cells is in the range of ≤ 10 - 25 pg/ml.
Species ReactivityHuman
BufferPBS
ReconstitutionThe lyophilized IL-6 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Stability and StorageThe lyophilized IL-6, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-6 should be stored in working aliquots at -20°C.
SynonymsIL6; HGF; HSF; BSF2; IL-6; IFNB2
DescriptionInterleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule. Mature human IL6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Human IL6 is equally active on mouse and rat cells. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/ IL-6 R but not from other cytokines that utilize gp130 as a co-receptor.
Protein SequenceMAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
Uniprot IDP05231
Protein RefSeqNP_000591
mRNA RefSeqNM_000600

Figures

Figure 1.
SDS-PAGE analysis of recombinant human IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Figure 2.
Proliferation assay with the mouse hybridoma cell line B9. The cells were stimulated using 2 different lots produced at ReliaTech, the WHO standard 89/548 and an IL-6 protein from a competitor. The cells were stimulated with increasing amounts of recombinant human IL-6.

Reference

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  8. Oversulfated heparins with low anticoagulant activity are strong and fast inhibitors of hepcidin expression in vitro and in vivo. Poli M et al., Biochem Pharmacol. 2014 Dec 1;92(3):467-75.
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