Human FGF-2 (basic) heat stable, 154 aa
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| Cat-Nr. | 100-450-HS |
| Size | 50 µg |
| Price | 345 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
| Length [aa] | 154 |
| Molecular Weight | 17.2 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (< 1EU/µg) |
| Biological Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml. |
| Species Reactivity | Human |
| Buffer | 5mM Tris, pH 7.6 + 150mM NaCl |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C. |
| Synonyms | Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin |
| Description | FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids. |
| Protein Sequence | AAGSITTLPA LPEDGGSGAF PPGHFKDPKL LYCKNGGFFL RIHPDGRVDG TRDKSDPFIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL YAIKNVTDEC FFFERLEENN YNTYRSRKYP SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS |
| Uniprot ID | P09038 |
| Protein RefSeq | NP_001997.5 |
| mRNA RefSeq | NM_002006.4 |
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