human FGF-2 (basic) 146 aa
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| Cat-Nr. | 100-411S |
| Size | 10 µg |
| Price | 95 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 146 |
| Molecular Weight | 16.4 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. |
| Species Reactivity | Human |
| Buffer | 5mM Tris, pH 7.6 + 150mM NaCl |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | FGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-3, Prostatropin |
| Description | FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic (146 a.a.) is a 16.4 kDa protein consisting of 146 amino acid residues. |
| Protein Sequence | PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
| Uniprot ID | P09038 |
| Protein RefSeq | NP_001997.5 |
| mRNA RefSeq | NM_002006.4 |
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