Human M-CSF
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| Cat-Nr. | 100-064 |
| Size | 10 µg |
| Price | 199 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 159 |
| Molecular Weight | 36.8 kDa |
| N Terminal Sequence | MEEVS |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | The biological activity was determined by the dose-dependent induction of proliferation in NFS60 cells (Murine myeloid Leukemia cell line). |
| Species Reactivity | Monkey, Mouse, Rat, Human |
| Buffer | PBS |
| Synonyms | CSF1; MCSF; CSF-1 |
| Description | Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Macrophage Colony Stimulating Factor Human Recombinant produced in E. coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD. M-CSF is purified by proprietary chromatographic techniques. |
| Protein Sequence | MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS |
| Uniprot ID | P09603 |
| Protein RefSeq | NP_000748.3 |
| mRNA RefSeq | NM_000757 |
Figures
SDS-PAGE analysis of recombinant human M-CSF. Samples were loaded in 18% SDS-polyacrylamide gel under reducing and non-reducing conditions and stained with Coomassie blue.
Dose-dependent stimulation of cell proliferation in NFS60 cells by recombinant human M-CSF. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
Reference
- KIF16B drives MT1-MMP recycling in macrophages and promotes co-invasion of cancer cells. S. Hey et al., Life Sci Alliance. 2023 Nov; 6(11): e202302158.
- Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. Cervero P. et al., Nat Commun. 2018 Feb 6;9(1):515.
- TGF-β1-dependent L1CAM expression has an essential role in macrophage-induced apoptosis resistance and cell migration of human intestinal epithelial cells. Schäfer H et al., Oncogene. 2013 Jan 10;32(2):180-9.
- Inflammatory Macrophages Induce Nrf2 Transcription Factor-dependent Proteasome Activity in Colonic NCM460 Cells and Thereby Confer Anti-apoptotic Protection. S. Sebens et al., J Biol Chem. 2011 Nov 25; 286(47): 40911–40921.
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