Human IL-12 p70
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| Cat-Nr. | 100-027S |
| Size | 2 µg |
| Price | 95 € |
| Source | CHO cells |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 503 |
| Molecular Weight | 65-70 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Determined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs. |
| Species Reactivity | Human |
| Synonyms | Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
| Description | IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 p70, derived from HEK293 cells, is a heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits (503 total amino acid residues). The calculated molecular weight of Human IL-12 p70 is 57.2 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of 65-70 kDa based on SDS-PAGE gel, under non-reducing conditions. |
| Protein Sequence | p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
| Uniprot ID | P29460 |
| Protein RefSeq | NP_002178.2 |
| mRNA RefSeq | NM_002187.2 |
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