Human Flt-3 Ligand
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| Cat-Nr. | 100-026-SC |
| Size | 50 µg |
| Price | 440 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & Coomassie stain |
| Length [aa] | 155 |
| Molecular Weight | 17.6 kDa |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. The expected ED50 for this effect is 0,5 – 2ng/ml. |
| Species Reactivity | Human |
| Synonyms | FLT3; FLK2; STK1; CD135; FLK-2 |
| Description | Flt3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells. Flt3-ligand produced in E. coli is a soluble non-glycosylated 17.6 kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein. |
| Protein Sequence | TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA |
| Uniprot ID | P49771 |
| Protein RefSeq | NP_001450.2 |
| mRNA RefSeq | NM_001459.3 |
Figures
SDS-PAGE analysis of recombinant human Flt3 Ligand. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
Flt3 Ligand induced proliferation of AML5 cells. AML5 cells were stimulated with increasing amounts of human Flt3 Ligand. The expected ED50 for this effect is 0,5 – 2ng/ml.
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