Human FGF-10
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| Cat-Nr. | 100-022 |
| Size | 25 µg |
| Price | 180 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE |
| Length [aa] | 170 |
| Molecular Weight | 19.3 kDa |
| N Terminal Sequence | MLGQDM |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF- receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg. |
| Species Reactivity | Chicken, Mouse, Human |
| Buffer | 0.5xPBS |
| Reconstitution | The lyophilized FGF-10 should be reconstituted in water to a concentration not lower than 50 μg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin. |
| Stability and Storage | Lyophilized samples are stable for more than six months at -20°C to -70°C. |
| Synonyms | FGF10; KGF2 |
| Description | Fibroblast Growth Factor-10 (FGF-10) is a heparin binding growth factor which stimulates the proliferation and activation of cells that express FGF receptors. FGF-10 is most related to KGF/FGF-7 and is expressed during development and preferentially in adult lungs. Human FGF-10 is a 19.3 kDa protein containing 170 amino acid residues. |
| Protein Sequence | MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS |
| Uniprot ID | O15520 |
| Protein RefSeq | NP_004456.1 |
| mRNA RefSeq | NM_004465 |
Figures
SDS-PAGE analysis of recombinant human FGF-10. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
FGF10-induced proliferation of 4MBr-5 rhesus monkey epithelial cell. (Rubin, J.S. et al., Proc. Natl. Acad. Sci. USA 86:802, 1989). The ED50 for this effect is typically 10-100 ng/mL.
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