Rabbit Anti-Rat VEGF-C
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| Cat-Nr. | 104-PA10 |
| Size | 200 µg |
| Price | 240 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Rat |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant rat dNdC-VEGF-C. Anti-rat VEGF-C IgG was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant rat VEGF-C (RT #R20-015) |
| Application | WB, E |
| Synonyms | vascular endothelial growth factor C; Vegfc |
| Description | VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed rat VEGF-C is a 16-18 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition. |
| Uniprot ID | O35757 |
| Protein RefSeq | NP_446105.1 |
| mRNA RefSeq | NM_053653.1 |
Figures
VEGF-C Sandwich-ELISA using the polyclonal anti-rat VEGF-C antibody [Cat# 104-PA10] as capture antibody and recombinant rat VEGF-C [Cat# R20-015] as standard. The Biotinylated rabbit anti-rat VEGF-C [Cat# 104-PABi10] was used as detection antibody.
Western Blot analysis of recombinant rat VEGF-C produced in insect cells using a polyclonal rabbit anti-rat VEGF-C antibody.
Reference
- Bone Marrow-Derived Mesenchymal Stem Cells Drive Lymphangiogenesis. L. Maertens et al., PLoS One. 2014; 9(9): e106976.
- Proteinuria Triggers Renal Lymphangiogenesis Prior to the Development of Interstitial Fibrosis. S. Yazdani et al., PLoS One. 2012; 7(11): e50209.
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