Rabbit Anti-Mouse Endomucin
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| Cat-Nr. | 103-PA49AG |
| Size | 50 µg |
| Price | 230 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant mouse soluble Endomucin (RT #S01-M64) |
| Application | WB, IF |
| Synonyms | Endomucin-1/2; Mucin-14 |
| Description | Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively |
| Uniprot ID | Q9ULC0 |
| Protein RefSeq | NP_001153166.1 |
| mRNA RefSeq | NM_001159694.1 |
Figures
Western Blot analysis of anti-mouse Endomucin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); lane 2: rm sEndomucin (250ng).
FACS analysis with primary mouse endothelial cells (SnoMec).
Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin (5µg/ml) [Cat# 103-PA49AG] and counter staining of nuclei with Dapi.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
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