Rabbit Anti-Human Lyve-1
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| Cat-Nr. | 102-PABi50 |
| Size | 50 µg |
| Price | 275 € |
| Category | Polyclonal Antibody |
| Label | Biotin |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Stabilizer/Carrier | BSA (50x), 0.02% sodium azide |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis). The antibody was purified via an antigen-affinity column and then biotinylated using a standard protocol. |
| Antigen | Recombinant human sLYVE-1 (RT #S01-028) |
| Application | WB, FC |
| Synonyms | LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1 |
| Description | LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule co-localizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. |
| Uniprot ID | Q9Y5Y7 |
| Protein RefSeq | NP_006682 |
| mRNA RefSeq | NM_006691 |
Figures
FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: Biotin-conjugated anti-human LYVE-1.
Reference
- Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair. Shin-Jeong Lee et al., Sci Rep. 2015; 5: 11019.
- Aberrant lymphatic development in euploid fetuses with increased nuchal translucency including Noonan syndrome. YM de Mooij et al., Prenat Diagn. 2011 Feb;31(2):159-66.
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