Rabbit Anti- GFP
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| Cat-Nr. | 102-PA84 |
| Size | 50 µg |
| Price | 130 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The anti-GFP antibody was generated by immunization of rabbits with highly purified recombinant GFP protein produced in E. coli. The anti-GFP antibodies were purified by affinity chromatography with immobilized Protein A. |
| Application | WB |
| Synonyms | green fluorescent protein |
| Description | Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues. The antibody was made against GFP from the jellyfish Aequorea Victoria using the whole native protein as an antigen. |
Figures
Western Blot analysis of recombinant GFP-His protein using a rabbit polyclonal anti-GFP antibody.
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