Rabbit Anti- His-tag
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| Cat-Nr. | 102-PA80 |
| Size | 50 µg |
| Price | 130 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The anti-His-tag antibody was generated by immunization of rabbits with different highly purified His-tagged proteins produced in insect cells. The anti-His-tag antibodies were then purified from sera by antigen-affinity chromatography using a His-peptide as matrix. |
| Application | WB |
| Synonyms | His-tag |
| Description | The His-tag antibody was generated by immunization of rabbits with several recombinant His-tagged proteins produced in insect cells and E. coli. It was antigen-affinity purified using a His-tagged protein as matrix. |
Figures
Western Blot analysis of conditioned supernatants from several recombinant His-tagged proteins produced in insect cells.
Western Blot analysis of cell lysate prepared from E.coli (Tuner[De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag using Reliatech's rabbit anti-His-tag antibody [Cat# 102-PA80]. As secondary antibody a AP-conjugated goat anti-rabbit antibody (Dianova) was used.
Western Blot analysis of conditioned supernatants (lane 2-3) from recombinant His-tagged proteins produced in insect cells and purified proteins produced in E. coli (lane 4-5).
Reference
- Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover. L. Grumet et al., J Biol Chem. 2016 Aug 19; 291(34): 17977–17987.
- Disruption of the methyltransferase-like 23 gene METTL23 causes mild autosomal recessive intellectual disability. M. Bernkopf et al., Hum Mol Genet. 2014 Aug 1;23(15):4015-23.
- Adiponutrin Functions as a Nutritionally Regulated Lysophosphatidic Acid Acyltransferase. M. Kumari et al., Cell Metab. 2012 May 2;15(5): 691–702.
- Characterization of rat thrombin-activatable fibrinolysis inhibitor (TAFI)--a comparative study assessing the biological equivalence of rat, murine and human TAFI. K. Hillmayer et al., J Thromb Haemost. 2006 Nov;4(11):2470-7.
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