Rabbit Anti-Human HGF
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| Cat-Nr. | 102-PA62S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Purified from sera of rabbits immunized with highly pure recombinant human HGF produced in insect cells. |
| Antigen | Recombinant human HGF (RT #300-011) |
| Application | WB |
| Synonyms | HGF; SF; HGFB; HPTA; F-TCF; DFNB39 |
| Description | HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. |
| Uniprot ID | P14210 |
| Protein RefSeq | NP_000592 |
| mRNA RefSeq | NM_000601 |
Figures
Western Blot analysis of recombinant human HGF [Cat# 300-010] using an anti-human HGF antibody [Cat# 102-PA62] directed against human HGF produced in insect cells. The SDS-PAGE was run under reducing conditions.
Functional ELISA with anti-human HGF [Cat# 102-PA62S]. Recombinant human HGF [Cat# 300-010] was coated with increasing amounts on a 96 well microtiter plate.
Western Blot analysis of recombinant human HGF [Cat# 300-010] using an anti-human HGF antibody [Cat# 102-PA62] directed against human HGF produced in insect cells. The SDS-PAGE was run under non-reducing conditions.
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