Rabbit Anti-Human Endocan/ESM-1
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| Cat-Nr. | 102-PA44S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | 0.5X PBS, pH 7.2 |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human ESM-1 (RT #300-062) |
| Application | WB, IF |
| Synonyms | Endocan, ESM1 |
| Description | Endocan, also known as endothelial cell specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is upregulated by TNFα, IL1β, or lipopolysaccharide and downregulated by IFNγ. The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte functionassociated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA1-mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported. |
| Uniprot ID | Q9NQ30 |
| Protein RefSeq | NP_008967.1 |
| mRNA RefSeq | NM_007036.4 |
Figures
Western Blot Analysis of anti-human Endocan/ESM-1. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); lane 2: rh ESM-1; lane 3: rm ESM-1
Immunofluorescence staining of cryo-sections of unfixed human foreskin with anti-human Endocan/EMS1 (dilution 1:100) [Cat# 102-PA44] and counter staining of nuclei with Dapi.
Note the specific green Endocan/ESM-1 signal in epidermis, connective tissue cells and vessels.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
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