Rabbit Anti-Human NRP-1
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| Cat-Nr. | 102-PA23AG |
| Size | 50 µg |
| Price | 230 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human NRP-1 (RT #S01-019) |
| Application | WB |
| Synonyms | Neuropilin receptor-1, VEGF165R, NRP, CD304; neuropilin |
| Description | Neuropilin-1 (NRP-1, CD304) is a 130-140 kDa type I transmembrane glycoprotein that regulates axon guidance and angiogenesis. The human NRP-1 contains a 623 aa extracellular domain (ECD) that shows 92-95% aa identity with mouse, rat, bovine and canine NRP-1. The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain. C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists. Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B; VEGF-E), PlGF-2, and the C-terminus of Sema3 bind the b1b2 region. NRP-1 and NRP-2 share 48% aa identity within the ECD and can form homo and hetero-oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as coreceptors with Plexins, mainly Plexin A3 and A4, to bind class III Semaphorins that mediate axon repulsion. However, only NRP-1 binds Sema3A, and only NRP-2 binds Sema 3F. Both are co-receptors with VEGFR-2 (KDR7Flk1) for VEGF165 binding. Sema 3A signaling can be blocked by VEGF165, which has higher affinity for NRP-1. NRP-1 is preferentially expressed in arteries during development or those undergoing remodeling. NRP-1 is also expressed on dendritic cells and mediates DC-induced T-cell proliferation |
| Uniprot ID | O14786 |
| Protein RefSeq | NP_003864.4 |
| mRNA RefSeq | NM_003873.5 |
Figures
Western Blot Analysis of anti-human NRP-1. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Lane 1: sNRP-1 conditioned supernatant from Sf9 cells. There is a strong cross reactivity with recombinant human sFLT4-Fc chimera but not with the other tested soluble receptors.
Immunofluorescence staining of NRP-1 (red) and CD31 (green) in human umbilical vein endothelial cells (HUVEC) with polyclonal rabbit anti-human NRP-1 [Cat# 102-PA23AG] and mouse anti-human CD31 #WM-59 [Cat# 101-M92]. As secondary antibodies goat anti-rabbit Cy3 / goat anti-mouse ALEXA Flour 488 [Dianova] were used. [5/2µg/ml; 400X]
Functional ELISA with anti-human NRP-1. Recombinant human soluble NRP-1 was coated with increasing amounts on a 96 well microtiter plate.
Immunofluorescence staining (green) of Neuropilin-1 (NRP-1) in primary human umbilical vein endothelial cells (HUVEC) with anti-human NRP-1 (5µg/ml) [Cat# 102-PA23AG] and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 (Dianova) was used 1:600.
FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
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