Rabbit Anti-Human VEGFR-1/Flt-1
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| Cat-Nr. | 102-PA20 |
| Size | 200 µg |
| Price | 240 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human soluble Flt-1 (D1-D5). Anti-human VEGFR-1/Flt-1 was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant human soluble Flt-1(D1-5) |
| Application | WB, E, IP |
| Synonyms | vascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1 |
| Description | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. |
| Uniprot ID | P17948 |
| Protein RefSeq | NP_001153392 |
| mRNA RefSeq | NM_001159920 |
Figures
Western analysis of recombinant human soluble VEGFR-1/Flt-1 using a polyclonal antibody directed against the extracellular domain of human VEGFR-1.
Reference
- Intracellular virion traffic to the endosome driven by cell type specific sialic acid receptors determines parvovirus tropism. T. Calvo-López et al., Front Microbiol. 2022; 13: 1063706.
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