Rabbit Anti-Human VEGFR-2/KDR
Slide this table
| Cat-Nr. | 102-PA18AG |
| Size | 50 µg |
| Price | 195 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human soluble extracellular domain of KDR (110 kDa) as the immunizing antigen. Anti-human VEGFR-2/KDR was purified by antigen-affinity chromatography with immobilized recombinant soluble VEGFR-2/KDR. The antibody is identical with the former described antibody R212 (see Lit.) |
| Antigen | Recombinant human KDR (D1-7) (RT #S01-001) |
| Application | WB, E, IP |
| Synonyms | vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor |
| Description | VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. |
| Uniprot ID | P35968 |
| Protein RefSeq | NP_002244.1 |
| mRNA RefSeq | NM_002253.2 |
Figures
Immunofluorescence staining of VEGFR-2/KDR (green) in primary human dermal lymphatic endothelial cells (HDLEC) with rabbit anti-human VEGFR-2 (5µg/ml) [Cat# 102-PA18AG] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:600.
Western Blot Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
IHC with paraffin sections of human Glioblastoma tissue with a polyclonal rabbit anti-human VEGFR-2/KDR antibody [5 µg/ml]. The sections were pretreated with 2 mMol HCL in the microwave. The secondary goat anti-rabbit antibody was used 1:500 [Dianova]. Blocking was performed with 10% goat serum.
The experiment was performed by Brigitte Maruschak, Dept. of Neuropathology, University Hospital Göttingen, Germany.
Double Immunofluorescence staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR [#101-M32], (B) rabbit anti-human KDR [B, #102-PA18AG], (C) DAPI and (D) merged.
Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) [Dianova] and goat anti-mouse PE [Santa Cruz].
FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Reference
- Lack of Evidence for the Direct Activation of Endothelial Cells by Adult Female and Microfilarial Excretory-Secretory Products. T. Weinkopff and P. Lammie, PLoS One. 2011; 6(8): e22282.
- The cytoprotective drug amifostine modifies both expression and activity of the pro-angiogenic factor VEGF-A. S. Dedieu et al., BMC Med. 2010; 8: 19.
All prices plus VAT + possible delivery charges