Rabbit Anti-Human Wnt-5a
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| Cat-Nr. | 102-PA141S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human Wnt-5a |
| Application | WB |
| Synonyms | Wnt-5a; Wnt5a; Wnt 5a; Wnt |
| Description | Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signalling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterised relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers. |
| Uniprot ID | P41221 |
| Protein RefSeq | NP_003383.2 |
| mRNA RefSeq | NM_003392.4 |
Figures
Western Blot analysis of recombinant human Wnt3a [Cat# 400-022] and Wnt5a [Cat# 400-023] using an anti-human Wnt5a polyclonal antibody. There is no cross reaction to recombinant human Wnt3a.
Western Blot analysis of recombinant human and mouse Wnt5a [Cat# 400-023; M30-023] using a rabbit anti-human Wnt5a antibody [Cat# 102-PA141] directed against recombinant human Wnt5a. As expected there is a cross reactivity with mouse Wnt5a. As secondary antibody an AP-conjugated goat anti-rabbit antibody was used.
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