Rabbit Anti-Human CD34
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| Cat-Nr. | 102-PA139AG |
| Size | 50 µg |
| Price | 230 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human sCD34 |
| Application | WB, IF |
| Synonyms | CD34 |
| Description | CD34 is a highly glycosylated type I membrane protein that is selectively expressed on hematopoietic stem cells and vascular endothelium. It has been widely used as a molecular marker for the identification, isolation, and manipulation of hemopoietic stem cells and progenitors. CD34 can function as a regulator of hemopoietic cell adhesion by mediating the attachment of stem cells to bone marrow stromal cells or other bone marrow components. The full length human CD34 is a 385 amino acid protein, consisting of a 31 amino acid signal sequence, a 74 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain and a 259 amino acid extracellular domain. Recombinant human sCD34 is a 258 amino acid polypeptide containing only the extracellular domain of the full length CD34 protein. |
| Uniprot ID | P28906 |
| Protein RefSeq | NP_001764.1 |
| mRNA RefSeq | NM_001773.2 |
Figures
Western Blot analysis of recombinant human sCD34 [Cat# S01-069] using an anti-human CD34 polyclonal antibody.
Immunofluorescence staining of human CD34 (green) [upper panel] and double staining with CD34 and human CD31 (red) in HDMECs with a polyclonal rabbit anti-human CD34 antibody [Cat# 102-PA139AG] and mouse anti-human CD31 [Cat# 101-M92] [lower panel]. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour 488 (1:600) [Dianova] and goat anti-mouse PE (1:400) [Santa Cruz].
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