Rabbit Anti-Human EGFR
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| Cat-Nr. | 102-PA06S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Purified from sera of rabbits immunized with highly pure recombinant human soluble EGFR produced in insect cells. |
| Antigen | Recombinant human soluble EGFR (RT #S01-040) |
| Application | WB; FC, IF |
| Synonyms | EGF receptor; EGFR; ERBB; HER1; mENA; ERBB1; PIG61 |
| Description | The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoprotein that has an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24aa putative signal peptide, a 621aa extracellular domain, a 23aa transmembrane domain, and a 542aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis. |
| Uniprot ID | P00533 |
| Protein RefSeq | NP_005219 |
| mRNA RefSeq | NM_005228 |
Figures
Immunofluorescence staining of human EGFR in the human epidermoid carcinoma cell line A431 with a polyclonal rabbit anti-human EGFR antibody [Cat# 102-PA06]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].
Functional ELISA: Binding of anti-human EGFR [Cat# 102-PA06] to recombinant human soluble EGFR [Cat# S01-040]. sEGFR was coated starting with 122pg/ml to 2000ng/ml (100µl/well). Detection was performed using a polyclonal rabbit anti-human EGFR (1µg/ml, 100µl/well) and a goat anti-rabbit Biotin conjugated secondary antibody.
FACS analysis with the human epidermoid carcinoma cell line A431.
Western Blot analysis of recombinant human soluble EGFR [Cat# S01-040] with a rabbit polyclonal EGFR antibody.
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