Rat Anti-Mouse VEGFR-3/FLT-4
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| Cat-Nr. | 103-M36 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#7A3) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The hybridoma was generated after fusion with cells from rat immunized with purified, insect cell-derived recombinant mouse soluble FLT-4 (120 kDa) as the immunizing antigen. Rat IgG1 antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | recombinant mouse soluble FLT-4 |
| Application | WB |
| Synonyms | vascular endothelial growth factor receptor-3; Flt4; Chy; Flt-4; VEGFR3; VEGFR-3 |
| Description | VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis. |
| Uniprot ID | P35917 |
| Protein RefSeq | NP_032055.1 |
| mRNA RefSeq | NM_008029 |
Figures
Immunofluorescence staining (green) of VEGFR-3/FLT-4 in primary mouse endothelial cells (SnoMec) with anti-mouse VEGFR-3/FLT-4 #7A3 (10μg/ml) [Cat# 103-M36] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:400.
Western analysis with VEGFR-3/FLT-4 expressing PAEC endothelial cells.
Reference
- Presence of lymphatics in a rat tendon lesion model. Tempfer H. et al., Histochem Cell Biol. 2015 Apr;143(4):411-9.
- Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.
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